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Hartford Athletic
New York Shockers
Sarasota Paradise
FC Naples
Miami FC
Naples United FC
New Jersey Alliance
Chattanooga Red Wolves
Asheville City
Greenville Triumph
West Chester United
Loudoun United
FC Motown
Westchester Flames
Texoma
Foro SC
Tulsa Athletics
FC Tulsa
AV Alta
Ventura County Fusion
Columbus Crew B
NY Renegades
Chattanooga
Corpus Christi FC
Appalachian FC
Knoxville troops
Richmond Kickers
Virginia Dream
International Miami B
Miami United
Sporting Kansas City(Dự bị)
Des Moines Menace
Lexington
Southern Indiana
Little Rock Street
Birmingham Legion
El Paso Locomotive FC
Harpo's FC
Real Monarchs
El Farolito SC
Monterey Bay FC
Inter San Francisco
Ballard Football Club
Spokane Velocity
Los Angeles FC II
Arizona FC
Los Angeles Galaxy II
Laguna United
Portland Hearts of Pine
Faialense
Long Island Rough Riders
Charlotte Independence
Forward Madison FC
Duluth
South Georgia Tormenta FC
Harbor City
Carolina Core
Soda City FC
Cincinnati II
NY Pancyprian Freedom
Flatirons Rush
Omaha
Tacoma Defiance
Washington Athletic